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. 2011 Apr 4;286(24):21535–21545. doi: 10.1074/jbc.M111.221143

FIGURE 4.

FIGURE 4.

Interaction between polySia-NCAM and BDNF. Top, titration of BDNF with STX(WT)-derived polySia-NCAM (7–900 ng as BSA); middle, titration of BDNF with STX(G421A)-derived polySia-NCAM (7–900 ng as BSA); bottom, titration of BDNF with STX(C621G)-derived polySia-NCAM (7–900 ng as BSA). The principle of this method was well described (28). The calculated EC50 values are indicated below each gel. +, anode region; −, cathode region; >, loaded position. The BDNF-polySia-NCAM complex is boxed by a dotted line.