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Fluorometric C7/C9 Analysis of NCAM-Fc
| Enzymes (STX) | C9-Neu5Acyla | C7Neu5Acyla | Neu5Aca | C9/(C7 + C9) |
|---|---|---|---|---|
| NCAM-Fc(wt) | 8.63 ± 2.78 | 13.6 ± 0.283 | 22.2 ± 2.96 | 0.39 ± 0.078 |
| NCAM-Fc(g421a) | 4.91 ± 1.14 | 11.0 ± 1.06 | 14.5 ± 1.98 | 0.31 ± 0.071b |
| NCAM-Fc(c621g) | 5.86 ± 0.643 | 12.1 ± 2.28 | 18.0 ± 2.12 | 0.34 ± 0.084 |
a Molar ratio analyzed with 1 mol of polySia-NCAM-Fc (〈Mr(avr)〉 = 200 kDa; see Fig. 2B).
b p < 0.05 (compared with wild type).
