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. 2011 Apr 29;286(24):21555–21564. doi: 10.1074/jbc.M111.224048

FIGURE 6.

FIGURE 6.

K13 interacts with A20. A, cell lysates (C.L.) prepared from BC3 cells expressing an empty vector or FLAG-tagged K13 were immunoprecipitated (I.P.) using control (C) or FLAG (F) beads, and the presence of interacting A20 and NEMO proteins was detected by Western blotting with the indicated antibodies. B, co-immunoprecipitation assay shows interaction of K13 with A20 in A20+/+ MEFs. The experiment was performed essentially as described for A. A tubulin blot shows equal protein loading. C, cell lysates (C.L.) prepared from BC1 and BC3 cells were immunoprecipitated using control antibody or 8F6 antibody raised against K13, and the presence of interacting A20 and IKK α/β proteins was detected by Western blotting with the indicated antibodies. D, A20 does not block the interaction between K13 and IKK complex subunits. 293T cells were transfected with an empty vector or a vector expressing FLAG-tagged K13, either alone or with A20, and a co-immunoprecipitation assay was performed essentially as described for A. A tubulin blot shows equal protein loading.