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. 2011 Apr 20;286(24):21577–21587. doi: 10.1074/jbc.M110.201137

FIGURE 3.

FIGURE 3.

Inhibition of CK2 by DRB decreases the instability of TTP protein. A–C, inhibition of CK2α by CK2 inhibitors or siRNA decreases the expression level of the TTP protein but does not affect the expression level of the TTP transcript. Colo320 cells were treated with CK2 inhibitor DRB or TBB at the indicated concentrations for 12 h or transfected with siCK2α or scRNA. TTP protein levels were determined by immunoblotting (IB) (A, top panel, and B) and TTP mRNA levels were determined by (A, bottom panel) semi-quantitative PCR, and real time PCR (C). D and E, DRB treatment decreases the stability of TTP protein. Colo320 cells were transfected with pcDNA6/V5-TTP, and at 24 h post-transfection cells were incubated with 10 μg/ml cycloheximide (CHX) in the presence or absence of DRB. D, cells were harvested at indicated times. and TTP protein levels were determined by immunoblotting with anti-V5 antibody. E, graph showing the expression levels of TTP protein. The expression levels of TTP protein in CHX-treated cells at 0 h were set to 1. The results are presented as the means ± S.D. of three independent experiments. (**, p < 0.01; ***, p < 0.001). ns, not significant.