FIGURE 2.

An N-terminal domain mediates ER localization of GADD34. Localization of WT GADD34-GFP, GADD34 lacking N-terminal 60 residues, (Δ1–60)-GFP, and N-terminal 10 amino acids, (Δ1–10)-GFP from GADD34 fused to GFP in COS7 cells, is shown. Cells were fixed as described under “Experimental Procedures” and stained with Hoechst (blue) to show nuclei, visualized for GFP fluorescence (green), and immunostained with anti-TRAPα (red). The merged images (Merge) are also shown.