FIGURE 6.

Fluorescence recovery after photobleaching of GFP-GADD34-expressing cells. A, representative COS7 cells expressing WT GFP-GADD34, GFP-(Δ1–60)-GADD34 (GFP-G34((Δ1–60)), GFP-(1–60)-GADD34 (GFP-G34(1–60)), and GFP-(V25R)-GADD34 (GFP-G34(V25R)) are shown. Two target areas, one subjected to photobleaching (solid circle) and one adjacent to the unbleached area (open circle), were monitored over time. B, the results for fluorescence recovery for 3–6 independent replicate experiments with error bars showing the S.E.