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. 2011 Apr 7;286(24):21732–21741. doi: 10.1074/jbc.M110.174680

FIGURE 4.

FIGURE 4.

Interaction between NVL2UD and nucleolin. A, summary of the binding assays between NVL2UD and various nucleolin fragments. B, in vitro binding assay with nucleolin fragments. Either GST (G) or GST-NVL2UD(1–74) (74) was incubated with E. coli cell lysates expressing various nucleolin fragments (in). The bound proteins were pulled down and separated using 15% SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. Asterisks denote the nucleolin fragments bound to NVL2UD(1–74). C, quantification of the nucleolin fragments bound to GST-NVL2UD(1–74). The signals corresponding to the nucleolin fragments in B were integrated and normalized by their molecular weights, using ImageJ. D, RNA is involved in the NVL2-nucleolin complex. An in vitro binding assay was conducted with nucleolin (R1234G) and wild-type or mutant NVL2UD(1–74) in the presence or absence of RNase A.