FIGURE 6.

Transgenic flies overexpressing Bβ2 or tws are more sensitive to oxidative injury. Newly eclosed Elav-gal4 (black bar), Elav>Bβ2 (gray bar), and Elav>tws (open bar) flies were exposed to drug-free or to 20 mm paraquat at 29 °C. A, survivorship was scored after 60 h. Compared with no drug treatment, administration of 20 mm paraquat reduced the survival of control Elav-gal4 by 6.8% and the survival of Bβ2 and tws transgenic flies was reduced by 16.5 and 15%, respectively. B, mobility assay was performed after 48 h. The mobility of control Elav-gal4 flies was not severely impaired in the presence of paraquat, but the climbing index was significantly lowered when Bβ2 or tws transgenic flies were challenged with paraquat. Data were expressed as mean ± S.D. values and analyzed by Student's t test; * indicates p < 0.01.