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CD spectrum of temperature-dependent unfolding of Drosophila myosin-18 proteins. Data were collected at 222 nm over a range of temperatures determined at Tm = 45.4 ± 0.1 °C for M-PDZ (●) and Tm = 46.3 ± 0.3 °C M-ΔPDZ (○). Both proteins were dialyzed into buffer containing 0.5 m KCl, 10 mm KH2PO4 (pH 7.2), 0.1 mm EGTA, 3 mm NaN3, and 1 mm DTT prior to the assay. The final concentration of M-PDZ used in the assay was 1.78 μm, and that of M-ΔPDZ was 2.85 μm. mdeg, millidegrees.
