FIGURE 2.

Effect of AdoMet (SAM), AdoHcy (SAH), and homocysteine on yeast growth. Overnight grown cultures of the yeast strains str4Δ were diluted to an OD (600 nm) of 0.1 in synthetic minimal media supplemented with all amino acids except methionine and serine (see under “Experimental Procedures”). Yeast cells were grown in the presence or absence of 600 μm AdoHcy, 600 μm AdoMet, or 5 mm homocysteine. Aliquots of yeast cells were taken, and the growth was monitored at indicated time points at 600 nm. The data represent the means ± S.D. (n = 3).