Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Apr 19;286(24):21779–21795. doi: 10.1074/jbc.M111.228072

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Comparison of differentially expressed genes in str4Δ cells in presence of homocysteine and its precursors. A, heat map of the expression profiles of differentially expressed genes in the presence of AdoHcy (SAH), AdoMet (SAM), AdoMet + AdoHcy (SAM + SAH), homocysteine, Hcy + AdoMet (Hcy + SAM) were compared and analyzed by hierarchical clustering using TIGR Multiexperiment Viewer software (MeV version 4.0). The cluster of groups with similar expression profiles are shown at top and left. B, metabolic map of differentially expressed genes in the presence of AdoMet + AdoHcy, homocysteine, and AdoHcy. The differentially expressed genes were mapped on yeast metabolic network using the web-based tool iPATH. The subset of nucleotide metabolism gene is highlighted in green, lipid metabolism in light blue, amino acid metabolism in purple, and the rest in red. C, expression of Met6 and CDC21 genes was determined by Northern blotting. The cells were grown in the presence and absence of homocysteine and its precursors, and RNA was isolated from these cells. The lower panels in each blot represent gel loading of total RNA. Images were acquired using PhosphorImager and quantified by ImageQuant software.