FIGURE 2.

NMR peak assignments of GroES in the unfolded state. A, two-dimensional ¹H-¹⁵N HSQC spectrum of GroES. Assignments are indicated in the figure. Measurement conditions are: 10 mg/ml GroES, 10 mm sodium phosphate buffer, 1.6 m Gdn-HCl, 10% (v/v) D₂O (pH 6.5) at 25 °C. B, SSP of GroES. Chemical shifts of Cα and Cβ of GroES were used to calculate the residue-specific SSP scores. A SSP score of 1 or −1 at a given residue position reflects a fully formed α- or β-structure, respectively, and 0 reflects a random coil.