FIGURE 3.

CB nanoparticles cause an increase in macrophage cell size. A, bright field images of CB nanoparticle-exposed cells were obtained 15 min after exposure and again after 24 h. Circles were drawn around representative cells from 10 fields and then used to calculate cellular area. B, fluorescent images of cells were obtained before CB nanoparticle exposure and again 24 h after exposure. Cells were loaded with cell tracker green 5-chloromethylfluorescein diacetate. Average cellular volume was calculated from the level of fluorescence per cell. C, transmission electron microscopy images of control and CB nanoparticle-exposed cells.