FIGURE 6.

WDR5 bound to SMC-marker gene promoter loci. A, stable cell lines containing either empty or Myc-WDR5 expression plasmid had comparable differentiation potential to the original A404 cell line. Control stable, stable A404 cell line containing empty pcDNA3.1 myc/his vector. B, WDR5 and Pitx2 bound to the SM α-actin and SM22α gene promoters as determined by ChIP assays. Stable cell lines containing either empty or Myc-WDR5 expression plasmids were processed to ChIP assays using Myc antibody and Pitx2 antibody. C, WDR5 and Pitx2 bound to the same physical chromatin within SM α-actin promoter. Sequential ChIP (1) assays were performed using Pitx2 antibody and Myc antibody in those stable cell lines as described in A.