FIGURE 5.

a, immunodetection of UCP2 in thymocytes isolated from WT and UCP2 null (KO) mice. Spleen lysate was used to confirm the electrophoretic mobility of UCP2. SDH was used as the loading control. UCP2 was detected with anti-UCP2 antibodies. b, impact of diamide on oligomycin-induced state 4 respiration in thymocytes from WT and UCP2 null (KO) mice. Cells were treated acutely for 15 min with oligomycin in the presence or absence of 100 μm diamide, and then oxygen consumption was tested. *, p < 0.05, n = 4, Student's t test. c, anti-biotin detection of glutathionylated UCP2. Kidney lysate from WT and UCP2 null (KO) mice was treated with or without BioGEE and then immunodetected with proteins modified by BioGEE using anti-biotin antibodies. Detection of UCP2 on the same gels was used to confirm the electrophoretic mobility of UCP2. Control reactions were performed in the absence of BioGEE. Error bars, S.D.