FIGURE 6.

Microinjection of a p115 inhibitory antibody results in multipolar spindles and binucleated cells. A, schematic showing the experimental procedure. HeLa cells stably expressing YFP-tagged α-tubulin were synchronized in S-phase with aphidicolin (see “Experimental Procedures”). After 5 h of release from the aphidocolin block, cells in prometaphase were injected with either p115 (7D1) or control IgG along with an injection marker. B, cells were then fixed at either 45 (a–h) or 240 min (i–p) postinjection and stained with Hoechst 33342 (blue). Number of injected, surviving cells is indicated in the figure (n) as well as the percentage of cells displaying the shown phenotype. Arrows, multipolar spindle triggered by microinjection of the p115 inhibitory antibody. Images are single sections acquired on an inverted microscope. Scale bars, 10 μm.