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. 2011 May 4;286(24):21937–21943. doi: 10.1074/jbc.M111.230706

FIGURE 3.

FIGURE 3.

Sequence conservation in the HC-Pro CPD. A, alignment of sequences from the Potyvirus genus. The black and gray shading represent 100 and 90% sequence similarity in 59 aligned sequences, respectively, eight of which are displayed. Similarity groups are defined as follows: S and T, D and E, K and R, and L, I, V, M, F, Y, and W. The secondary structure elements and residue numbers are indicated for TuMV HC-Pro. The red arrow points to the self-cleavage site. The catalytic dyad residues are shaded in green. The P1′ residue Gly458, the putative S1′ residue Trp379 and their equivalents that are invariant in other sequences are shaded in magenta. The P4 residue Tyr455, the S4 residues Val409 and His411 and their equivalents invariant in other sequences are shaded in yellow. ZYMV, zucchini yellow mosaic virus; PPV, plum pox virus; PVY, potato virus Y; LMV, lettuce mosaic virus; TVMV, tobacco vein mottling virus; ClYVV, clover yellow vein virus. B, conservation in the Potyviridae family. Five potyviral HC-Pro proteins (TuMV, TEV, ZYMV, PPV, and PVY, not shown) and all available homologs from other genera of the Potyviridae family are aligned. Residues with 100 and 90% conservation in these sequences are shaded in black and gray, respectively. The genera are indicated. AgMV, agropyron mosaic virus; HoMV, hordeum mosaic virus; RGMV, ryegrass mosaic virus, BrSMV, brome streak mosaic virus; WSMV, wheat streak mosaic virus; ONMV, oat necrotic mottle virus; WEqMV, wheat eqlid mosaic virus; SPMMV, sweet potato mild mottle virus; BlVY, blackberry virus Y; TriMV, Triticum mosaic virus; WYMV, wheat yellow mosaic virus; BaYMV, barley yellow mosaic virus; BaMMV, barley mild mosaic virus; OMV, oat mosaic virus. C, conserved surface at two opposite orientations. Residues at least 90% conserved in the genus Potyvirus as defined in A are colored yellow, and those at least 90% conserved across the Potyviridae family as defined in B are colored red. The corresponding ribbon representations are shown in parallel. Residue Asp420 is shown as spheres.