FIGURE 4.
Interaction of YCF48 and Sll0933. A, membrane proteins from wild-type cells were solubilized with 1.3% β-dodecyl maltoside and co-immunoprecipitated using anti-YCF48 antiserum or preimmune serum (pre). FT, of the flow-through containing unbound proteins; W, of the supernatant from the last washing step; E, one-half of the eluted fraction containing co-immunoprecipitated proteins. B, two-dimensional Blue native/SDS-PAGE of wild-type, ycf48−, and ins0933 membranes (each fraction is equivalent to 20 μg of chlorophyll) was performed as described previously (5). RCa and RCb are RC complexes a and b (these complexes are not defined in text), RC47 is the PSII core complex lacking CP43, and RCC1 is the monomeric PSII core complex. The positions of size markers are indicated at the top. The indicated proteins were detected using the respective antibodies.