TABLE 2.
Oligonucleotides used in this study
| Oligo name | Purpose | Sequence (5′->3′) |
| lacZCC101A | Recombineering A→C primer | cacccgagtgtgatcatctggtcgctggggaattagtcaggccacggcgctaatcacgacgcgctgtatc |
| lacZCC102A | Recombineering G→A primer | cacccgagtgtgatcatctggtcgctggggaatgggtcaggccacggcgctaatcacgacgcgctgtatc |
| lacZCC103A | Recombineering G→C primer | cacccgagtgtgatcatctggtcgctggggaatcagtcaggccacggcgctaatcacgacgcgctgtatc |
| lacZCC104A | Recombineering G→T primer | cacccgagtgtgatcatctggtcgctggggaatgcgtcaggccacggcgctaatcacgacgcgctgtatc |
| lacZCC105A | Recombineering A→T primer | cacccgagtgtgatcatctggtcgctggggaatgtgtcaggccacggcgctaatcacgacgcgctgtatc |
| lacZCC106A | Recombineering A→G primer | cacccgagtgtgatcatctggtcgctggggaataagtcaggccacggcgctaatcacgacgcgctgtatc |
| lacZCC107A | Recombineering +1G primer | ctcgatccttcccgcccggtgcagtatgaaggcggggggccgacaccacggccaccgatattatttgc |
| lacZCC108A | Recombineering −1G primer | ctcgatccttcccgcccggtgcagtatgaaggggggcggagccgacaccacggccaccgatattatttgc |
| lacZCCnewA | Recombineering +2CG primer | cacggccaccgatattatttgcccgatgtacgcgcgtggatgaagaccagcccttcccggctgtgc |
| lacZCC109A | Recombineering −2CG primer | cacggccaccgatattatttgcccgatgtacgcgcgcgcgtggatgaagaccagcccttcccggctgtgc |
| lacZCC110A | Recombineering +1A primer | agcccttcccggctgtgccgaaatggtccatcaaaaatggctttcgctacctggagagacgcgcccgctg |
| lacZCC111A | Recombineering −1A primer | agcccttcccggctgtgccgaaatggtccatcaaaaaaatggctttcgctacctggagagacgcgcccgctg |
| lacZCCCon1 | PCR for cc strains forward primer | atggtgccaatgaatcgtctg |
| lacZCCCon2 | PCR for cc strains reverse primer | ccgccctgtaaacggggatactgac |
| lacZccseq1 | Sequencing for cc strains forward primer | tgatccgcgctggctaccggc |
| lacZccseq2 | Sequencing for cc strains reverse primer | acgaaacgcctgccagtattt |
| lacZdup11A | Recombineering +11 dup | gttgcagtgcacggcagatacacttgctgatgcggtgctgatgcggtgctgattacgaccgctcacgcgtggcagcatcag |
| lacZdup11Con1 | PCR for +11 dup forward primer | ctttcacagatgtggattggc |
| lacZdup11Con2 | PCR for +11 dup reverse primer | cctaatccgagccagtttaccc |
| lacZdup11seq1 | Sequencing for +11 dup forward primer | gataaaaaacaactgctgacgccgc |
| lacZdup11seq2 | Sequencing for +11dup reverse primer | cagtttacccgctctgctac |
| lacZshpQP3A | Recombineering QP3 primer A | gaatggcgaatggcgctttgcctggtttccgcggcaccggaagcggtgccggaaagctggctggagtgcg |
| lacZshpQP4A | Recombineering QP4 | gaatggcgctttgcctggtttccggcaccggaagcggtgccgcggaaagctggctggagtgcgatcttcctg |
| newQP5oligo | Recombineering QP5 | cgcagcctgaatggcgaatggcgctttgccagcttctcttccggcaccggaagcggtgccggaaagctggctgga |
| QP6ultra | Recombineering QP6 | gcgaatggcgctttgccagctttccggcaccggaagcggtgccggaatct |
| lacZQPcon1 | PCR for QP3/4/5/6 | cctggcgttacccaacttaatcgcc |
| lacZQPcon2 | PCR for QP3/4/5/6 | gtgggaacaaacggcggattgaccg |
| lacZQPseq1 | Sequencing primers QP3/4/5/6 | ttgcagcacatccccctttcgccag |
| lacZQPseq2 | Sequencing primers QP3/4/5/6 | cgtaatgggataggtcacgttggtg |