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Gat1 inhibits filament formation during mating. Filamentation assays on V8 and MS mating media (pH 5.0) showed that filament formation was enhanced in unilateral gat1Δ crosses in comparison to the wild-type crosses. Filamentation was enhanced even further in a bilateral gat1Δ cross. Complementation of the H99α gat1Δ and KN99a gat1Δ mutants with the GAT1 gene restored wild-type mating in all tested crosses (data not shown).
