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. 2011 Jul 1;85(1):162–168. doi: 10.4269/ajtmh.2011.10-0469

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Figure 3. — Phylogenetic relatedness among Canadian, California, and Colorado Calbertado virus nonstructural protein 5 (NS5) gene sequences. Nucleotide sequences of the NS5 gene segment obtained from mosquito pools from Manitoba (MB), Colorado (CO), Alberta (AB), British Columbia (BC), and California (CA) were aligned by using ClustalW, and the resulting dendogram was generated by using MEGA version 4.0. GenBank accession numbers of Calbertado virus NS5 sequences are 52–48_(MB) GU952252, 44–13_(MB) GU952253, 5901_(CO) GU952254, 5890_(CO) GU952255, 10_(AB) GU952256, 95_(AB) GU952257, 90_(SK) GU952258, 11_(AB) GU952259, 1055_(AB) GU952260, 27_(AB) GU952261, 10073_(BC) GU952262, 10067_(BC) GU952263, 10057_(BC) GU952264, 10051_(BC) GU952265, 10047_(BC) GU952266, 10026_(BC) GU952267, AFSB5045(CA) GU952268, AFSB5050(CA) GU952269, AFSB5037(CA) GU952270, 42_(SK) GU952271, and 43_(SK) GU952272. Scale bar indicates nucleotide substitutions per site.