Table 2.
Antibodies used for immunocytochemistry
| Antibody | Antibody type | Target | Source | Staining |
|---|---|---|---|---|
| E06 | Mouse (mAB) | Oxidized phospholipids | Palinski et al., 1996 | 10 µg/ml |
| E014 | Mouse (mAB) | Malondialdehyde–lysine | Palinski et al., 1996 | 0.4 µg/ml |
| MDA2 | Mouse (mAB) | Malondialdehyde–lysine | Palinski et al., 1990 | 1 : 1000 |
| 8-OHdG | Goat (polyAB) | 8-Hydroxy 2-deoxy Guanosine | Abcam, ab10802 | 1 : 1000; E |
| CD3 | Rabbit (polyAB) | T-cell antigen | Dako, A0452 Lot128 | 1 : 2000; E |
| IBA-1 | Rabbit (polyAB) | Ionized calcium binding adaptor molecule 1 | Courtesy of Dr Sonja Forss-Petter/Japan | 1 : 3000; E |
| CD68 | Mouse (mAB) | CD68 110-kD transmembrane glycoprotein | Dako, M0814 | 1 : 100; E |
| HLA-D | Mouse (mAB) | Human leucocyte antigen-DP, DQ, DR | Dako, M0775 | 1 : 100; C |
| TPPP/p25 | Rat (polyAB) | Tubulin polymerization promoting protein | Produced by Gergö Botond | 1 : 3000; E |
| CNPase | Mouse (mAB) | 2',3'-cyclic nucleotide 3' phosphodiesterase | Sternberger Monoclonals, SMI91 | 1 : 2000; E |
| MOG Y10 and Z12 | Mouse (mABs) | Myelin oligodendrocyte glycoprotein | Piddlesden et al., 1991 | 1 : 100; C |
| PLP | Mouse (mAB) | Proteolipid protein | Serotec, MCA 839 G | 1 : 1000; E |
| APP | Mouse (mAB) | Alzheimer amyloid precursor protein | Chemicon, MAB 348 | 1 : 1000; C |
| SY | Rabbit (polyAB) | Synaptophysin | Epitomics YE269, Burlingame, CA, USA | 1 : 100; E |
| SMI 32 | Mouse (mAB) | Non-phosphorylated 200 kDa neurofilament | Sternberger Monoclonals SMI32 | 1 : 1000; E |
| SMI 311 and 312 | Mouse (mAB) | Neurofilament | Sternberger Monoclonals SMI 311 and 312 | 1 : 18000; E |
| GFAP | Rabbit (polyAB) | Glial fibrillary acidic protein (intermediate filament of mature astrocytes) | Dako, Z0334 Lot 096 | 1 : 3000; E |
AB = antibody; C = citrate buffer; E =ethylenedinitrilotetraacetic acid buffer.