Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1992 Apr 11;20(7):1559–1565. doi: 10.1093/nar/20.7.1559

A physical and genetic map of the Spiroplasma citri genome.

F Ye 1, F Laigret 1, J C Whitley 1, C Citti 1, L R Finch 1, P Carle 1, J Renaudin 1, J M Bové 1
PMCID: PMC312238  PMID: 1349741

Abstract

A physical and genetic map of the Spiroplasma citri genome has been constructed using several restriction enzymes and pulsed field gel electrophoresis. A number of genes were subsequently localized on the map by the use of appropriate probes. The genome size of the spiroplasma estimated from restriction fragments is close to 1780 kbp, the largest of all Mollicutes studied so far. It contains multisite insertions of Spiroplasma virus 1 (SpV1) sequences. The physical and genetic map of the S. citri genome shares several features with that of other Mollicutes, especially those in the Mycoplasma mycoides cluster. This supports the finding that S. citri and these Mycoplasma spp. are phylogenetically related.

Full text

PDF
1559

Images in this article

1561Image
on p.1561
1562Image
on p.1562
1563Image
on p.1563
1563Image
on p.1563

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 8. Microbiol Rev. 1990 Jun;54(2):130–197. doi: 10.1128/mr.54.2.130-197.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bautsch W. Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques. Nucleic Acids Res. 1988 Dec 23;16(24):11461–11467. doi: 10.1093/nar/16.24.11461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chevalier C., Saillard C., Bové J. M. Organization and nucleotide sequences of the Spiroplasma citri genes for ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase, pyruvate kinase, and an unidentified protein. J Bacteriol. 1990 May;172(5):2693–2703. doi: 10.1128/jb.172.5.2693-2703.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cocks B. G., Pyle L. E., Finch L. R. A physical map of the genome of Ureaplasma urealyticum 960T with ribosomal RNA loci. Nucleic Acids Res. 1989 Aug 25;17(16):6713–6719. doi: 10.1093/nar/17.16.6713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Colman S. D., Hu P. C., Bott K. F. Mycoplasma pneumoniae DNA gyrase genes. Mol Microbiol. 1990 Jul;4(7):1129–1134. doi: 10.1111/j.1365-2958.1990.tb00687.x. [DOI] [PubMed] [Google Scholar]
  6. Colman S. D., Hu P. C., Litaker W., Bott K. F. A physical map of the Mycoplasma genitalium genome. Mol Microbiol. 1990 Apr;4(4):683–687. doi: 10.1111/j.1365-2958.1990.tb00638.x. [DOI] [PubMed] [Google Scholar]
  7. Engel J. N., Ganem D. A polymerase chain reaction-based approach to cloning sigma factors from eubacteria and its application to the isolation of a sigma-70 homolog from Chlamydia trachomatis. J Bacteriol. 1990 May;172(5):2447–2455. doi: 10.1128/jb.172.5.2447-2455.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grau O., Laigret F., Carle P., Tully J. G., Rose D. L., Bové J. M. Identification of a plant-derived mollicute as a strain of an avian pathogen, Mycoplasma iowae, and its implications for mollicute taxonomy. Int J Syst Bacteriol. 1991 Oct;41(4):473–478. doi: 10.1099/00207713-41-4-473. [DOI] [PubMed] [Google Scholar]
  9. Hesse J. E., Wieczorek L., Altendorf K., Reicin A. S., Dorus E., Epstein W. Sequence homology between two membrane transport ATPases, the Kdp-ATPase of Escherichia coli and the Ca2+-ATPase of sarcoplasmic reticulum. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4746–4750. doi: 10.1073/pnas.81.15.4746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Krause D. C., Mawn C. B. Physical analysis and mapping of the Mycoplasma pneumoniae chromosome. J Bacteriol. 1990 Sep;172(9):4790–4797. doi: 10.1128/jb.172.9.4790-4797.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Muto A., Andachi Y., Yuzawa H., Yamao F., Osawa S. The organization and evolution of transfer RNA genes in Mycoplasma capricolum. Nucleic Acids Res. 1990 Sep 11;18(17):5037–5043. doi: 10.1093/nar/18.17.5037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Neimark H. C., Lange C. S. Pulse-field electrophoresis indicates full-length Mycoplasma chromosomes range widely in size. Nucleic Acids Res. 1990 Sep 25;18(18):5443–5448. doi: 10.1093/nar/18.18.5443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ohkubo S., Muto A., Kawauchi Y., Yamao F., Osawa S. The ribosomal protein gene cluster of Mycoplasma capricolum. Mol Gen Genet. 1987 Dec;210(2):314–322. doi: 10.1007/BF00325700. [DOI] [PubMed] [Google Scholar]
  14. Pyle L. E., Corcoran L. N., Cocks B. G., Bergemann A. D., Whitley J. C., Finch L. R. Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes. Nucleic Acids Res. 1988 Jul 11;16(13):6015–6025. doi: 10.1093/nar/16.13.6015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pyle L. E., Finch L. R. A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci. Nucleic Acids Res. 1988 Jul 11;16(13):6027–6039. doi: 10.1093/nar/16.13.6027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pyle L. E., Finch L. R. Preparation and FIGE separation of infrequent restriction fragments from Mycoplasma mycoides DNA. Nucleic Acids Res. 1988 Mar 25;16(5):2263–2268. doi: 10.1093/nar/16.5.2263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pyle L. E., Taylor T., Finch L. R. Genomic maps of some strains within the Mycoplasma mycoides cluster. J Bacteriol. 1990 Dec;172(12):7265–7268. doi: 10.1128/jb.172.12.7265-7268.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Reed K. C., Mann D. A. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 1985 Oct 25;13(20):7207–7221. doi: 10.1093/nar/13.20.7207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Renaudin J., Aullo P., Vignault J. C., Bové J. M. Complete nucleotide sequence of the genome of Spiroplasma citri virus SpV1-R8A2 B. Nucleic Acids Res. 1990 Mar 11;18(5):1293–1293. doi: 10.1093/nar/18.5.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Robertson J. A., Pyle L. E., Stemke G. W., Finch L. R. Human ureaplasmas show diverse genome sizes by pulsed-field electrophoresis. Nucleic Acids Res. 1990 Mar 25;18(6):1451–1455. doi: 10.1093/nar/18.6.1451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rogers M. J., Steinmetz A. A., Walker R. T. The nucleotide sequence of a tRNA gene cluster from Spiroplasma meliferum. Nucleic Acids Res. 1986 Apr 11;14(7):3145–3145. doi: 10.1093/nar/14.7.3145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Samuelsson T., Guindy Y. Nucleotide sequence of a Mycoplasma mycoides RNA which is homologous to E. coli 4.5S RNA. Nucleic Acids Res. 1990 Aug 25;18(16):4938–4938. doi: 10.1093/nar/18.16.4938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  24. Su C. J., Baseman J. B. Genome size of Mycoplasma genitalium. J Bacteriol. 1990 Aug;172(8):4705–4707. doi: 10.1128/jb.172.8.4705-4707.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., Van Etten J. A phylogenetic analysis of the mycoplasmas: basis for their classification. J Bacteriol. 1989 Dec;171(12):6455–6467. doi: 10.1128/jb.171.12.6455-6467.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wenzel R., Herrmann R. Physical mapping of the Mycoplasma pneumoniae genome. Nucleic Acids Res. 1988 Sep 12;16(17):8323–8336. doi: 10.1093/nar/16.17.8323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Williamson D. L., Renaudin J., Bové J. M. Nucleotide sequence of the Spiroplasma citri fibril protein gene. J Bacteriol. 1991 Jul;173(14):4353–4362. doi: 10.1128/jb.173.14.4353-4362.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Woese C. R., Stackebrandt E., Ludwig W. What are mycoplasmas: the relationship of tempo and mode in bacterial evolution. J Mol Evol. 1984;21(4):305–316. doi: 10.1007/BF02115648. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES