Table 1.
Viability of cells dispersed from biofilms during continuous antifungal drug treatment under flow conditionsa
| Drug | Concn (μg/ml) | Nonviable dispersed cells (%) |
||||
|---|---|---|---|---|---|---|
| 1 h | 3 h | 6 h | 12 h | 24 h | ||
| FLC | 1,024 | 16.5 ± 4.8 | 31.3 ± 3.2 | 48.7 ± 3.8 | 47.0 ± 4.2 | 66.8 ± 2.3 |
| 256 | 9.5 ± 6.2 | 16.2 ± 2.6 | 26.5 ± 2.7 | 27.3 ± 4.9 | 37.5 ± 2.9 | |
| 64 | 5.5 ± 2.2 | 11.6 ± 1.4 | 15 ± 5.6 | 16.9 ± 4 | 14.5 ± 7.7 | |
| AMB | 16 | 13.8 ± 3.9 | 15.7 ± 0.0 | 34.5 ± 0.7 | 50 ± 14.1 | 64.2 ± 6 |
| 4 | 0.0 ± 0.0 | 11.8 ± 3.8 | 14.2 ± 8.0 | 46.6 ± 0.0 | 63.4 ± 10.3 | |
| 1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 3.8 ± 0.0 | 44.0 ± 0.0 | 38.0 ± 8.9 | |
| 0.25 | 10.9 ± 4.6 | 9.0 ± 1.4 | 6.1 ± 1.2 | 46.1 ± 8.8 | 40.8 ± 6.5 | |
| CSP | 0.25 | 23.6 ± 1.8 | 68.5 ± 4.0 | 74.5 ± 7.7 | 92.2 ± 0.3 | 98.2 ± 0.1 |
| 0.125 | 1.3 ± 1.8 | 51.7 ± 2.4 | 40.0 ± 4.0 | 81.2 ± 1.7 | 81.4 ± 1.9 | |
| 0.06 | 6.6 ± 0.0 | 2.6 ± 3.7 | 22.2 ± 4.7 | 34.3 ± 4.4 | 46.7 ± 2.3 | |
| 0.03 | 2.0 ± 1.0 | 1.6 ± 2.3 | 3.8 ± 5.3 | 11.4 ± 7.7 | 17.4 ± 2.2 | |
a
Results are percentages of nonviable cells and are expressed as averages and standard deviations for a single experiment conducted in duplicate. The experiments were repeated with similar results.