Table 4.
Proportion of livers, kidneys, and spleens of infected mice that were culture negative after 24, 72, and 120 h of therapy with DAMB alone or in combination with the Mycograb C28Y variant
| Treatment arm | Proportion of organ samples culture negative at the indicated time (h) posttreatment |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Liver |
Kidney |
Spleen |
|||||||
| 24 | 72a | 120 | 24 | 72a | 120 | 24 | 72a | 120 | |
| No-treatment control | 0/5 | 0/10b | 0/5 | 0/10 | 0/5 | 0/10 | |||
| Vehicle control | 0/5 | 0/13 | 0/5 | 0/13 | 0/5 | 0/13 | |||
| Myc-C28Y control | 0/5 | 0/13 | 0/5 | 0/13 | 0/5 | 0/13 | |||
| DAMB alone | 0/5 | 1/5 | 4/5c | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 1/5 |
| DAMB + vehicle | 0/5 | 1/5 | 4/8c | 0/5 | 0/5 | 0/8 | 0/5 | 0/5 | 1/7d |
| DAMB + Myc-C28Y, 0.15 mg/kg/day | 0/5 | 2/5 | 5/7c,e | 0/5 | 0/5 | 0/7e | 0/5 | 0/5 | 2/7e |
| DAMB + Myc-C28Y, 1.5 mg/kg/day | 0/5 | 2/5 | 5/8c | 0/5 | 0/5 | 0/8 | 0/5 | 0/5 | 0/8 |
| DAMB + Myc-C28Y, 15 mg/kg/day | 0/5 | 2/5 | 6/8c | 0/5 | 0/5 | 0/8 | 0/5 | 0/5 | 0/8 |
In the animals in the no-treatment control, vehicle control, and Myc-C28Y control groups that were scheduled for sacrifice at the 72- and 120-h time points, animals were culled 48 h after fungal inoculation because of severe, progressive infection. They were included in the 72-h time point in this table.
In groups that were treated with i.v. vehicle or Myc-C28Y fragment, three additional mice were included to increase the probability that at least five mice were evaluable at the last time point, if they survived their infections. This accounts for the higher number of animals in these groups being evaluated at the last time point.
The portions of sterile cultures in these groups were significantly different from the no-treatment control, the vehicle control, and the Myc-C28Y control arms.
The livers and kidneys of 8 animals were quantitatively cultured in the DAMB plus vehicle group. However, only 7 spleens were evaluated, because 1 of animals in this group did not have a spleen.
One animal in the AMB plus Myc-C28Y at 0.15 mg/kg/day group did not receive a full dose of antibody fragment on day 4 of treatment and was excluded from evaluation.