Fig. 2.

Anoxic growth of B. pseudomallei via nitrate respiration. (A) Anoxic growth of B. pseudomallei cultures, as assessed by CFU determination, upon the addition of 25 mM sodium nitrate after 2 days of anaerobic conditions. (B) Reduction of nitrate to nitrite by anaerobic B. pseudomallei. Anaerobic cultures either were injected with water or nitrate or were left untreated and were assayed for nitrite production by using the Griess reaction. Error bars represent standard deviations for three independent cultures per treatment.