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Ammonia accumulation and toxicity in anaerobic B. pseudomallei cultures. (A) Ammonia accumulation in LBG at pH 6.0 (open bars), 6.5 (striped bars), or 7.0 (filled bars). Error bars represent standard deviations for three independent cultures per time point. (B) Anaerobic survival of B. pseudomallei over the course of 1 week in LBG with no added ammonia (circles), 5 mM ammonia (squares), or 20 mM ammonia (triangles).
