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. 2011 Jul;55(7):3399–3405. doi: 10.1128/AAC.01801-10

Fig. 1.

Fig. 1.

Regulation of rsmZ and rsmY expression in the presence or absence of AZM. (A) The β-galactosidase activities of an rsmZ-lacZ transcriptional fusion carried by pME3331 (squares) and growth (triangles) were determined in P. aeruginosa PAO1 in the absence (filled symbols) or presence (empty symbols) of 2 μg/ml of AZM. The experiments were done in triplicate; average values ± standard deviations are shown. (B) The β-galactosidase activities of an rsmY-lacZ transcriptional fusion carried by pME7311 (squares) and growth (triangles) were determined in P. aeruginosa PAO1 in the absence (filled symbols) and presence (empty symbols) of 2 μg/ml of AZM. The experiments were done in triplicate; average values ± standard deviations are shown. (C) Northern blot analysis of RsmZ and RsmY RNA in the absence (−) and presence (+) of AZM. Total RNA (3 μg) was prepared from cultures in the late exponential phase (OD600 ≥ 3). 5S rRNA was used as a loading control.