Fig. 6.

Preincubation of SB105 and SB105_A10 with viruses does not affect HSV infectivity. HSV-1 and HSV-2 aliquots (10⁴ PFU) were incubated at either 4 or 37°C for various lengths of time with no dendrimers (open squares) or 25 μM SB105 (closed triangles), SB105_A10 (opened triangles), or SB104 (closed circles). After incubation, the samples were diluted to reduce the dendrimer concentration below that which inhibits HSV replication (0.02 μM), and the virus was titrated on Vero cells. Plaques were microscopically counted and the mean plaque counts for each peptide concentration expressed as PFU/ml on a log₁₀ scale. The data shown are means ± SD (error bars) from three independent experiments performed in duplicate.