Fig. 7.

SB105_A10 binds to cell surface heparan sulfate. Vero cell monolayers were left untreated or treated with heparinase I or heparitinase I (2.5 U/ml) for 1 h at 37°C, washed with medium, and chilled at 4°C. Next, SB105_A10-FAM (5 μM) was added alone or in the presence of a 10-fold molar excess of SB105_A10 or heparin (10 μg/ml) for a further 1 h at 4°C. Cells were then fixed, counterstained with Evans blue, and examined by fluorescence microscopy. The experiment was repeated three times, and representative images are presented.