Table 1.

MICs, MLVA types, and point mutations of partial sequences of 23S rRNA and ribosomal protein L3 genes recovered in Spain between 2008 and 2009, isolate subset A^a

Isolate	MLVA type	MICd (μg/ml)					Point mutation in 23S rRNA gene atb:											L3 mutationc
		TIA	VAL	LINC	TYLO	TYLV	A2031	G2032	G2057	A2058	A2059	G2087	C2146	C2362	G2365	G2535	C2611
RSp1	3	32	32	32	1,024	8				T						A
RSp2	3	32	32	64	2,048	4				T			T		C			N148S
RSp3	3	32	8	128	512	4		A		T						A
RSp7	3	8	4	>64	>128	>32				T	G
RSp9	3	4	2	16	>128	4				T
RSp19	3	8	4	64	>128	4				T
RSp12	9	64	>32	256	1,024	16		A		T				T
RSp13	9	16	8	32	1,024	32				T				T
RSp4	12	16	4	128	1,024	4			A	G							T
RSp10	12	4	2	>64	>128	2			A	G							T
RSp20	12	8	>4	64	>128	8			A	G							T
RSp18	13	8	4	16	>128	16				T
RSp5	14	32	2	32	512	4				T		T
RSp15	22	4	2	32	>128	4				T
RSp6	24	4	2	>64	>128	4		A		T
RSp8	24	8	2	>64	>128	2		A		T
RSp11	24	8	2	>64	>128	16	T	A		T								S149T
RSp14	24	4	1	>64	>128	2	T	A		T								S149T
RSp17	24	8	2	>64	>128	4		A		T
RSp16	45	4	>4	64	>128	8		A		T

^aSubset A includes 20 B. hyodysenteriae isolates for which the tiamulin MICs were ≥4 μg/ml.

^b23S rRNA positions numbered according to Escherichia coli (J01695) numbering.

^cRibosomal protein L3 amino acids numbered according to B. pilosicoli (AF114845) numbering.

^dTIA, tiamulin; VAL, valnemulin; LINC, lincomycin; TYLO, tylosin; TYLV, tylvalosin.