Table 2.
DNA sequences of oligonucleotides used in real-time RT-PCR in this study
| Oligonucleotidea | Sequence | Reference |
|---|---|---|
| Full pmrCAB-F | 5′-GCATCATAAAAAGATTGTAGTCAC-3′ | This study |
| Full pmrCAB-R | 5′-GCGATTTGTATTCATCGTTTTGAG-3′ | This study |
| pmrA-F | 5′-ATGACAAAAATCTTGATGATTGAAGAT-3′ | This study |
| pmrA-R | 5′-TTATGATTGCCCCAAACGGTAG-3′ | This study |
| pmrB-F | 5′-GTGCATTATTCATTAAAAAAAC-3′ | This study |
| pmrB-R | 5′-TCACGCTCTTGTTTCATGTA-3′ | This study |
| pmrB2-F | 5′-GGTTCGTGAAGCTTTCG-3′ | This study |
| pmrB2-R | 5′-CCTAAATCGATTTCTTTTTG-3′ | This study |
| pmrC-F | 5′-ATGTTTAATCTCATTATAGCCA-3′ | This study |
| pmrC-R | 5′-TTAGTTTACATGGGCACAA-3′ | This study |
| pmrC2-F | 5′-GGTTGTTATTGAAGAAAGTAT-3′ | This study |
| pmrC2-R | 5′-TCAATCCAAGTCACTTGGTAAC-3′ | This study |
| PMRBINTFW | 5′-CGCTCAAGGTATAGTCAG-3′ | This study |
| PMRBINTRV | 5′-CAGTAGGCTCGACCATAC-3′ | This study |
| PMRBEXTFW | 5′-CAGTGTCATCTTAGGTTG-3′ | This study |
| PMRBEXTRV | 5′-CGTAGTGATCTGGATCGG-3′ | This study |
| RT-PCR pmrA-F | Same as that of pmrA-F | This study |
| RT-PCR pmrA-R | 5′-CCATCATAGGCAATCCTAAATCCA-3′ | This study |
| RT-PCR pmrB-F | 5′-GAACAGCTGAGCACCCTTTAA-3′ | This study |
| RT-PCR pmrB-R | 5′-ACAGGTGGAACCAGCAAATG-3′ | This study |
| RT-PCR pmrC-F | 5′-CTCTTTACGCTTTGTTTTATGGAC-3′ | This study |
| RT-PCR pmrC-R | 5′-GTAAAAAGTAAAACACCGACCA-3′ | This study |
| RT-PCR 16S rRNA-F | 5′-TCAGCTCGTGTCGTGAGATG-3′ | 19 |
| RT-PCR 16S rRNA-R | 5′-CGTAAGGGCCATGATG-3′ | 19 |
| blaOXA-51like-F | 5′-TAATGCTTTGATCGGCCTTG-3′ | 46 |
| blaOXA-51like-R | 5′-TGGATTGCACTTCATCTTGG-3′ | 46 |
a
F, forward oligonucleotide; R, reverse oligonucleotide.