Fig. 3.
(A) Dose-response experiment. Shown is a dot blot analysis for detecting the presence of the blaOXA-24 gene in OMVs released from A. baumannii clinical isolate AbH12O-A2. Blot A, 10 μl of purified plasmid pMMA2 as a positive control; blot B, 10 μl of the OMV suspension (see concentration in Table 1); blot C, 50 μl of the OMV suspension; blot D, 100 μl of the OMV suspension; blot E, 200 μl of the OMV suspension; blot F, 100 μl of the OMV suspension from A. baumannii ATCC 17978 mock transformed as a negative control (equal amount of OMVs with respect to that in blot E). All samples were concentrated to a final volume of 10 μl before the experiment, and 5 μl of each sample was analyzed by dot blotting. (B) Dot blot analysis to detect the presence of the blaOXA-24 gene in OMVs released from A. baumannii clinical isolates AbH12O-A2 and AbH12O-CU3 (blots A and B, respectively) and from A. baumannii ATCC 17978 transformed with OMVs from clinic isolates AbH12O-A2 and AbH12O-CU3 (blots C and D, respectively). The mock-transformed A. baumannii strain ATCC 17978 (blot E) was used as a negative control. Equal amounts of OMVs of each sample were analyzed in the dot blots.