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Gene deletion by specialized transduction: organization of mshA gene loci in mc2155 wild type (WT) and the ΔmshA::hyg and ΔmshA unmarked mutants. The central part of target genes was deleted and replaced by the γδres-sacB-hyg-γδres cassette, which was subsequently removed utilizing the phage phAE201 expressing the γδ-resolvase. Only the first and last ∼50 bp of the target gene was maintained in the genome of desired unmarked mutants.
