Table 1.

Oligonucleotides used for generation of allelic exchange substrates

Primer	Sequence	Restriction enzyme used
mshA
LL	5′-TTTTTTTTCCATAAATTGGATCTGCTCGGCCGCACGCTC-3′	Van91I
LR	5′-TTTTTTTTCCATTTCTTGGTGCGCCAGCGGAGAGGTGTG-3′	Van91I
RL	5′-TTTTTTTTGCATAGATTGCCGTTCCGGACGCCGGTTCTC-3′	BstAPI
RR	5′-TTTTTTTTGCATCTTTTGCCGGCGTCCTGGCTGTACTTG-3′	BstAPI
mshB
LL	5′-TTTTTTTTGCATAAATTGCGGCGACGACAACACCGTGAC-3′	BstAPI
LR	5′-TTTTTTTTGCATTTCTTGCCCGGTGGTCAGGGTCTCGTC-3′	BstAPI
RL	5′-TTTTTTTTGCATAGATTGCGGGCCACGGGATTCCCGAGG-3′	BstAPI
RR	5′-TTTTTTTTGCATCTTTTGCGCGCCTGAGTCGCTCGAGTC-3′	BstAPI
mshC
LL	5′-TTTTTTTTCCATAAATTGGATTCCGGCGGGCACCGATAC-3′	Van91I
LR	5′-TTTTTTTTCCATTTCTTGGGTCGAAGAGGCGCAGCGCAG-3′	Van91I
RL	5′-TTTTTTTTCCATAGATTGGACGCGCTGTCCTACGGTGGG-3′	Van91I
RR	5′-TTTTTTTTCCATCTTTTGGTGTGCGCGCTGCTCGATCTG-3′	Van91I
mshD
LL	5′-TTTTTTTTGCATAAATTGCGCGGCTGACGAGCAGGTGAG-3′	BstAPI
LR	5′-TTTTTTTTGCATTTCTTGCTCAGCGCGCGAATCTCTGCC-3′	BstAPI
RL	5′-TTTTTTTTCCATAGATTGGACCGGAAGTTGGGGTTCGAG-3′	Van91I
RR	5′-TTTTTTTTCCATCTTTTGGCGAAGCCCTTCTCGACGTAG-3′	Van91I
mca
LL	5′-TTTTTTTTCCATAAATTGGGTGATAGCCGCCGTTCTCGC-3′	Van91I
LR	5′-TTTTTTTTCCATTTCTTGGCTTGCTGGACTCGTCGTCCG-3′	Van91I
RL	5′-TTTTTTTTCCATAGATTGGGCCGACCGAGGAGTTCGAG-3′	Van91I
RR	5′-TTTTTTTTCCATCTTTTGGTGGCGCTTGTCCTCGTCACC-3′	Van91I