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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2011 Jul;70(7):578–587. doi: 10.1097/NEN.0b013e3182204414

Figure 2.

Figure 2

Double label fluorescence microscopy in progressive supranuclear palsy (PSP). (A-C) A large number of PSP tangles show both ppRbser807 (A, green) and AT8 (B, red) marked with white arrowheads and there are also many AT8-positive neurites that do not contain ppRbser807 (C, merged image). (D-F) There are a few neurons that are strongly immunoreactive for ppRbser807 (D, green), but lack AT8 (E, red) (white arrow) and evident in merged image (F). White arrowheads indicate neurons with colocalization. (G-I) The same pattern is found using an antibody specific for ppRbS249/T252 (G, green). Many neurites only stain with antibody AT8 (H, red); some PSP tangles display only ppRbS249/T252 and not phosphorylated tau (I, merged image, white arrow); some PSP tangles show colocalization (white arrowheads). Scale bars = 50 μm.