Table 2.
BD reagents used and peripheral blood lymphocyte subsets measured in the study
| Reagent (BD) | Subset | Anchor marker | Surface markera | Subset |
|---|---|---|---|---|
| Multitest CD3/CD19/CD45/CD16 + 56 | 19 | CD45 | 3−/19 | B cell |
| 16/56 | CD45 | 3−/natural killer | Natural killer cell | |
| Multitest CD3/CD8/CD45/CD4 | 4 | CD45 | 3/4 | Helper T cell |
| 8 | CD45 | 3/8 | Cytotoxic T cell | |
| 3 | CD45 | T cells | ||
| Multitest CD45RA/CD62L/CD3/CD4 | 4/45RA/62L | CD4 | 45RA/62L | Naive helper T cell (CD62L+) |
| 4/45RA | CD4 | 45RA/62L+62L− | Naive helper T cell | |
| Multitest CD45RA/CD62L/CD3/CD8 | 8/45RA/62L | CD8 | 45RA/62L | Naive cytotoxic T cell (CD62L+) |
| 8/45RA | CD8 | 45RA/62L+62L− | Naive cytotoxic T cell | |
| Multitest CD4/CD38/CD3/HLA-DR | 4/DR/38 | CD4 | DR/38 | Activated helper T cell |
| 4/DR | CD4 | DR/38+38− | Activated helper T cell (DR+) | |
| CD4 | 38/DR+DR− | Activated helper T cell (CD38+) | ||
| Multitest CD8/CD38/CD3/HLA-DR | 8/DR/38 | CD8 | DR/38 | Activated cytotoxic T cell |
| 8/DR | CD8 | DR/38+38− | Activated cytotoxic T cell (DR+) | |
| CD8 | 38/DR+DR− | Activated cytotoxic T cell (CD38+) | ||
| Multitest CD45RA/CD45RO/CD3/CD4 | 3/4/45RO | CD3 | 4/45RO | Memory helper T cells |
| 3/4/45RA | CD3 | 4/45RA | Naive helper T cells | |
| Multitest CD45RA/CD45RO/CD3/CD8 | 3/8/45RO | CD3 | 8/45RO | Memory cytotoxic T cells |
| 3/8/RA | CD3 | 8/45RA | Naive helper T cells | |
| αβ/γδ/CD3 | γδ T cell | CD3 | γδ+/αβ− | γδ T cell |
| αβ T cell | CD3 | γδ−/αβ+ | αβ T cell |
a
The presence of a surface marker on a cell is assumed unless the marker is followed by a superscript minus sign. The presence of both plus and minus signs indicates a total cell population.