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(A) 2D gel electrophoresis of the eluted BHUP2 protein. The circle around the spot is the eluted protein. (B) Western blotting of the eluted protein separated on a 2D gel, hybridized with sera from patients with parasitologically confirmed VL. 2D gel electrophoresis of the eluted protein revealed 3 to 4 protein spots, and out of these protein spots, only two of them showed antibody reactivity against the eluted protein.
