Fig. 1.

Characterization of r-Listeria ΔactA prfA* expressing SIV Gag or anthrax PA. Shown are Western blot analyses results for protein expression in the culture supernatants of r-Listeria ΔactA prfA* expressing Gag (A) and r-Listeria ΔactA prfA* expressing PA (B). The supernatants were collected after 16 to 18 of culturing at 30°C. Monoclonal anti-PA or anti-Gag Ab was used in the blot assay. The expressed full-length protein sizes can be judged based on the markers on the left of each blot. The nonrecombinant Listeria ΔactA prfA* strain (Lm) served as a negative control in the assay.