Innate immune function in patients with recurrent meningitis (RM) compared to patients with recurrent genitalis (RG) and healthy seropositive controls (HC). (A) PBMCs from RG (striped bar, n = 7) and RM (filled bar, n = 8) patients were cultured overnight in the presence of live HSV-2. The percentage of gated NK cells positive for IFN-γ as determined by intracellular FACS analysis is shown. P = 0.06. (B) PBMCs from healthy seropositive controls (unfilled bars, n = 10) and from RM patients (filled bars, n = 10) were freshly stained for indicated TLRs. TLR median fluorescence intensity (MFI) of gated pDC as determined by intracellular FACS analysis is shown. The Mann-Whitney U test was used for statistical analysis. *, P < 0.05. (C) IFN-α production by PBMCs from healthy seropositive controls (unfilled bars, n = 10) and from RM patients (filled bars, n = 10) cocultured overnight with or without poly(I:C) as indicated. The Mann-Whitney U test was used for statistical analysis. *, P < 0.05.