IgG binding to DENV NS3 proteins. Sera from PIV/LAV-, LAV/LAV-, or PBS-vaccinated macaques (four in each group) were collected at days 0, 60, 90, 210, and 261 and analyzed for binding to the DENV proteome microarray. The figure shows IgG binding to DENV-1 NS3 (upper left), binding to DENV-2 NS3 (upper right), binding to DENV-3 NS3 (lower left), and binding to DENV-4 NS3 (lower right). The y axis represents the geometric mean of the average pixel value for all four subjects in each group. Error bars represent the standard error of the mean in each group. Statistical significance (*) was assigned to each group compared to results for day 0 (0d) (P ≤ 0.05).