Comparative status of GM-CSF- and IL-4-differentiated iDCs from myeloid cells obtained from CaCx patients and healthy individuals. Monocytes were isolated from blood specimens obtained from CaCx patients (n = 6) and healthy individuals (n = 6) and differentiated with GM-CSF plus IL-4 for 5 days. The statuses of CD1a and CD14 in iDCs before maturation between CaCx patients and healthy individuals were compared. (A) Forward scatter (FSC)/side scatter (SSC) dot plot of DCs; (B, D) acquisition of CD1a phenotypes, depicted by a representative figure (B) and a bar diagram showing the percentage of positive cells (D); (C, E) loss of CD14 phenotypes, depicted by a representative figure (C) and a bar diagram showing the percentage of positive cells (E). *, P < 0.01 (CD1a cells in comparison to healthy cells); **, P < 0.001 (CD14 cells in comparison to healthy cells).