Fig. 5.

Induction of tumor cell cytotoxicity by antigen-primed T cells. MACS-purified T cells obtained from CaCx patients (n = 6) and healthy individuals (n = 6) were cocultured with irradiated iDCs and mDCs for 48 h. mDCs-LPS and mDCs-NLGP were pulsed with HeLa cell extract before use in coculture. CTLs generated under these conditions were used to lyse antigen-positive HeLa cells and antigen-negative U937 cells after coculturing at an effector/target (E/T) ratio of 1:10. Bar diagrams represent the mean cytotoxicity values ± SDs obtained from six individual experiments. *, P < 0.001 (iDCs versus NLGP/LPS DCs from healthy individuals/CaCx patients).