Table 4.
Example of a single specimen that was cross-reactive in the MAC ELISA for DENV and WNV and subsequently tested by the PRNT90a
| Serum | PRNT90 titer of antigen to: |
||||
|---|---|---|---|---|---|
| DEN1 | DEN2 | DEN3 | DEN4 | WNV | |
| Nondepleted | >640 | >640 | >640 | >640 | 80 |
| Depleted | 64 | 256 | >512 | 16 | <16 |
a
The results presented for DENV1 to -4 and WNV demonstrate the difference in PRNT titers in IgG-depleted serum and nondepleted serum from the specimen. Using IgG depletion identified DENV as the infecting virus for this sample (in bold). The starting dilutions for the sample were 1:16 for IgG-depleted serum and 1:40 for nondepleted serum. (The sample endpoint was not determined due to insufficient sample volume.).