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IgG antibody titers (A) and serum IgG1 and IgG2a levels (B) were determined by ELISA. The mice were immunized three times at 2-week intervals, and sera were collected at 1-week intervals after each immunization. Results are shown as means ± standard deviations (SD). *, P < 0.05 between the indicated groups; **, P < 0.01 between the indicated groups; ***, P < 0.001 between the indicated groups.
