Table 3.
Recovery of bacteria from the mammary glands in the rSip-ClfA, inactivated-bacterium, and PBS groups
| Group | Immunogen/challengea | Bacteria in mammary glands [log10 (CFU)/ml] | Reduction of bacteria [log10 (CFU)/ml]b | Significance (P) relative to control |
|---|---|---|---|---|
| 1 | rSip-ClfA/S. agalactiae | 5.269 ± 0.748 | 2.926 | <0.0001 |
| 2 | Inactivated S. agalactiae/S. agalactiae | 7.658 ± 0.503 | 0.537 | 0.0163 |
| 3 | PBS/S. agalactiae | 8.195 ± 0.175 | ||
| 4 | rSip-ClfA/S. aureus | 6.413 ± 0.539 | 2.878 | <0.0001 |
| 5 | Inactivated S. aureus/S. aureus | 8.727 ± 0.814 | 0.564 | 0.1269 |
| 6 | PBS/S. aureus | 9.291 ± 0.800 |
a
The immunized mice were challenged with 5 × 105 CFU of S. agalactiae or S. aureus per gland.
b
Reduction of the number of bacteria in mammary gland [log10 (CFU)/ml] is a measure of protective immunity and here is relative to the value for mice immunized with PBS.