Fig. 6.

Inhibition of JNK MAPK abolished the activation of NF-κB and AP-1, whereas ERK pathway inhibition enhanced PGN-induced activation of NF-κB. An EMSA was performed with nuclear proteins isolated from murine macrophages pretreated for 30 min with or without specific inhibitors of MAPKs ERK (PD98059; 50 μM) and JNK (SP600125; 50 μM) and then further stimulated with PGN for 1 h. (A) Biotin-labeled NF-κB oligonucleotides probes were incubated in the presence of nuclear extract. (B) Biotin-labeled AP-1 oligonucleotides probes were incubated in the presence of nuclear extract. The data are representative of three independent experiments. The lower panel shows the densitometry analysis of the EMSA bands.