Table 1.
Primers used in modified multiple-locus variable-number tandem-repeat analysis (MMLVA)a
| Marker (PM) | Primer | Primer sequence (5′ to 3′) | Reference |
|---|---|---|---|
| A6Cd (1) | A6-F | FAM-TTAATTGAGGGAGAATGTTAAA | 19 |
| A6-R | AAATACTTTTCCCACTTTCATAA | 19 | |
| B7Cd (1) | B7-F | VIC-TTAATACTAAACTAACTCTAACCAGTAA | Modified from reference 19 |
| B7-NAP7/8-F | VIC-TTAATATTAAACTAACTCTAACCAGTAA | Modified from reference 19 | |
| B7-universal-R | TTATATTTTATGGGYATGTTAAA | Modified from reference 19 | |
| C6Cd (2) | C6-630, 91, and 96-F | VIC-GTTTAGAATCTACARCATTATTTGA | Modified from reference 19 |
| C6-NAP7/8-F | ATTTAGAATCTATACTATTATTTGA | Modified from reference 19 | |
| C6-R | ATTGGAATTGAATGTAACAAAA | 19 | |
| C6-NAP7/8-R | AGCGGAATTGAATGTAACAAAA | Modified from reference 19 | |
| E7Cd (2) | E7-F | FAM-TGGAGCTATGGAAATTGATAA | 19 |
| E7-R | CAAATACATCTTGCATTAATTCTT | 19 | |
| E7-NAP7/8-R | CAAATACATCTTGCACTAGTTCTT | Modified from reference 19 | |
| G8Cd (1) | G8-F | NED-TGTATGAAGCAAGCTTTTTATT | 19 |
| G8-R | ACCAAAAATTTCTAACCCAAC | 3 | |
| G8-NAP7/8-R | ACCAAAATTTTCTAACCCAAC | Modified from reference 3 | |
| tpi (3) | tpi-F | FAM-AAGAAGCTACTAAGGGTACAAA | 9 |
| tpi-R | CATAATATTGGGTCTATTCCTAC | 9 | |
| tcdA (3) | tcdA-F | FAM-AGATTCCTATATTTACATGACAATAT | 9 |
| tcdA-R | GTATCAGGCATAAAGTAATATACTTT | 9 | |
| tcdB (3) | tcdB-F | FAM-GGAAAAGAGAATGGTTTTATTAA | 9 |
| tcdB-R | ATCTTTAGTTATAACTTTGACATCTTT | 9 | |
| cdtB (3) | cdtB-F | FAM-CTTAATGCAAGTAAATACTGAG | 18 |
| cdtB-R | AACGGATCTCTTGCTTCAGTC | 18 | |
| tcdC deletion (2) | tcdC-F | FAM-AAGCTATTGAAGCTGAAAATC | 1 |
| tcdC-R | GCTAATTGGTCATAAGTAATACC | 1 |
a
Modifications to published primers are indicated as underlined nucleotides. Three multiplex reactions were used simultaneously under identical cycling conditions. Primer mix (PM) combinations and forward (F) and reverse (R) primers are indicated. Fluorophores are named next to each relevant primer sequence.