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Electron microscopic analysis showing the presence of capsular material in Y. pestis cultures. Both WT CO92 and its Δcaf mutant were grown on SBA plates at 37°C for 48 h. The bacilli then were fixed and examined with a Philips 201 electron microscope at 60 kV. The arrow indicates the shedding of the capsular material for the WT CO92, but no such capsular material was seen surrounding the Δcaf mutant.
