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. 2011 May;49(5):1750–1757. doi: 10.1128/JCM.02348-10

Fig. 3.

Fig. 3.

Representative multiplex PCR with alpha and beta mixes separated on 2% agarose. (A) Lane 1, mixture of PCR products amplified with the alpha primer sets on HS4A, HS15, HS41, HS53, HS10, HS6, HS3, and HS2 DNAs; lane 2, 100-bp NEB DNA standard; lane 3, mixture of PCR products amplified with the beta primer sets on HS4B, HS1, HS42, HS17, HS23, and HS44 DNAs. Amplicons were separated on 2% agarose as described in Materials and Methods. The position of each CPS-specific amplicon is shown. (B) Typical PCR products obtained with primer mixes alpha and beta. Lane 1, alpha primer sets on HS3 DNA; lane 2, alpha primer sets on HS4 DNA; lane 3, alpha primer sets on HS6 DNA; lane 4, 100-bp NEB DNA standard; lane 5, alpha primer sets on HS10 DNA; lane 6, alpha primer sets on HS15 DNA; lane 7, alpha primer sets on HS41 DNA; lane 8, alpha primer sets on HS53 DNA; lane 9, 100-bp NEB DNA standard; lane 10, PCR beta primer sets on HS1 DNA; lane 11, beta primer sets on HS4/13/64 DNA; lane 12, beta primer sets on HS8 DNA; lane 13, beta primer sets on HS23 DNA.